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28/03/2007 - Neuroprotective effect by Dammishimgyu-herbal acupuncture against H(2)O(2)-induced apoptosis in human neuroblastoma, SH-SY5Y cells.

Country: Korea

Institute: TKM Research Group, Kyung Hee University, Seoul 130-701, Korea.

Author(s): Park HJ, Kim Y, Kim H, Ha E, Park H, Yoon S, Kim MJ, Choi S, Ryu Y, Park HK, Hong M.

Journal: Neurol Res. 2007;29 Suppl 1:93-7.


BACKGROUND: The free radical is involved in neuronal cell death in human neurodegenerative disease. Dammishimgyu (DMSG)-herbal acupuncture has been used to treat neurological disorders in Korea. The present study was aimed to investigate the neuroprotective effect of DMSG-herbal acupuncture against H(2)O(2)-induced apoptosis in human neuroblastoma cell line, SH-SY5Y.

METHODS: The neuroprotective effect of DMSG-herbal acupuncture on H(2)O(2) induced apoptosis was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, reverse transcription-polymerase chain reaction (RT-PCR), western blots and nitrite assay.

RESULTS: In this study, 100 muM H(2)O(2)-treated cells decreased the cell viability with apoptotic features and increased the production of nitric oxide (NO). However, 0.1% DMSG treatment after exposure to 100 muM H(2)O(2) inhibited both H(2)O(2)-stimulated mRNA and protein expressions of BCL2-associated X protein (BAX) and caspase 3 apoptosis-related cysteine peptidase (CASP3). In addition, 0.1% DMSG treatment inhibited the increased NO production induced by H(2)O(2).

CONCLUSION: These results suggest that DMSG-herbal acupuncture shows protective effect against H(2)O(2)-induced neuronal damage.